Fig. 2

Optimization of the LAMP reaction for the detection of V. parahaemolyticus. M: 100 bp marker; 1: negative control. a Effect of Mg2+ concentrations on the LAMP reaction: 2–10 Mg2+ was 2, 4, 6, 8, 10, 12, 14, 16 and 18 mM, respectively. b Effect of dNTPs concentrations on the LAMP reaction: 2–11 dNTP was 0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6 and 1.8 mM, respectively. c Effect of betaine concentrations on the LAMP reaction: 2–8 betaine was 0, 0.2, 0.4, 0.6, 0.8, 1.0 and 1.2 M, respectively. d Effect of the ratio of outer and inner primers on the LAMP reaction: 2–6 the ratio was 1:1, 1:2, 1:4, 1:6 and 1:8, respectively. e Effect of temperature on the LAMP reaction: 2–7 the temperature was 53, 55, 58, 60, 63 and 65°C, respectively. f Effect of reaction time on the LAMP reaction: 2–7 the amplification time was 15, 30, 45, 60, 75 and 90 min, respectively