Fig. 6
The sensitivity of PCR for detection of a V. parahaemolyticus ATCC 33846 genomic DNA. M 100 bp marker; 1 positive control; 2 22.5 ng test tube-1; 3 2.25 ng test tube-1; 4 225 pg test tube-1; 5 22.5 pg test tube-1; 6 2.25 pg test tube-1; 7 225 fg test tube-1; 8 negative control; b V. parahaemolyticus ATCC 33846 in pure cultures. M 100 bp marker; 1 positive control; 2 2.39 × 105 CFU mL-1; 3 2.39 × 104 CFU mL-1; 4 2.39 × 103 CFU mL-1; 5 2.39 × 102 CFU mL-1; 6 2.39 × 101 CFU mL-1; 7 2.39 CFU mL-1; 8 2.39 × 10-1 CFU mL-1; 9 negative control; and c V. parahaemolyticus ATCC 33846 in seeded shrimp samples. M 100 bp marker; 1 positive control; 2 8.9 × 106 CFU g-1; 3 8.9 × 105 CFU g-1; 4 8.9 × 104 CFU g-1; 5 8.9 × 103 CFU g-1; 6 8.9 × 102 CFU g-1; 7 8.9 × 101 CFU g-1; 8 8.9 × 100 CFU g-1; 9 8.9 × 10-1 CFU g-1; 10 negative control