Table 2 Occurrence of alfalfa seed-borne fungi using standard moist blotter (SMB), deep-freezing blotter (DFB) and alkaline seed-bed methods

Acremonium strictum

6.70

0.033 ± 0.03

20

0.13 ± 0.07 (0.5-1)

26.7

0.23 ± 0.1 (0.5-2)

26.7

0.17 ± 0.079 (0.5-1)

Alternaria alternata

100

10.33 ± 3.65 (0.5-55)

86.70

9.47 ± 2.55 (1–28.5)

93.30

5.76 ± 1.8 (0.5-25.5)

80

4.93 ± 1.68 (1–34.5)

Aspergillus flavipes

80

1.57 ± 0.4 (0.5-5.5)

40

1.3 ± 0.6 (0.5-9)

53.3

0.73 ± 0.25 (0.5-3)

21.8

0.9 ± 0.32 (1–1.5)

Aspergillus flavus

86.67

3.27 ± 0.69 (0.5-8)

46.70

0.7 ± 0.31 (0.5-4)

53.33

1.27 ± 0.48 (0.5-6)

60

1.47 ± 0.41 (1–4.5)

Aspergillus glaucus

26.70

0.27 ± 0.14 (0.5-2)

53.30

0.27 ± 0.067 (0.5-1)

53.30

1.67 ± 0.54 (0.5-5)

53.30

1.63 ± 0.62 (0.5-5.5)

Aspergillus nidulans

80

2.4 ± 0.78 (0.5-10)

33.30

1.53 ± 0.1 (0.5-13)

60

1.87 ± 0.73 (0.5-10)

66.70

1.93 ± 0.69 (0.5-9.5)

Aspergillus niger

66.67

3.7 ± 0.98 (0.5-8)

46.70

0.57 ± 0.21 (0.5-2.5)

66.67

1.9 ± 0.43 (1–5)

60

1.76 ± 0.49 (1–4.5)

Aspergillus ochraceus

33.30

1.97 ± 0.96 (0.5-10)

33.30

1.47 ± 0.78 (0.5-9.5)

60

0.73 ± 0.25 (0.5-4.5)

73.3

0.9 ± 0.32 (0.5-6.5)

Aspergillus tamarii

73.30

1.63 ± 0.39 (0.5-4)

13.30

0.1 ± 0.072

26.70

0.17 ± 0.079 (0.5-1)

26.70

0.27 ± 0.14 (0.5-2)

Cephalosporium acremonium

13.30

0.1 ± 0.07 (0.5-1)

60

1.33 ± 0.076 (1.5-3)

73.30

1.63 ± 0.13 (0.5-6)

60

1.23 ± 0.41 (0.5-4)

Chaetomium sp.

6.70

0.33 ± 0.03

6.70

0.067 ± 0.067

20

0.1 ± 0.053

13.30

0.67 ± 0.045

Cladosporium sp.

100

21.7 ± 5.8 (0.5-63)

53.30

3.97 ± 1.84 (0.5-13.5)

60

6.63 ± 3.04 (0.5-35.5)

40

7.3 ± 3.30 (0.5-37.5)

Colletotrichum trifolii

13.30

0.06 ± 0.04

13.30

0.13 ± 0.35

13.30

0.5 ± 0.035 (3–4.5)

13.30

0.37 ± 0.25 (2.5-3)

Cunninghamella sp.

-

-

13.30

0.1 ± 0.072

-

-

-

-

Curvularia lunata

-

-

-

-

6.70

0.03 ± 0.033

6.70

0.03 ± 0.033

Drechslera tetramera

26.70

0.2 ± 0.095 (0.5-1)

20

0.13 ± 0.08 (0.5-1)

13.30

0.1 ± 0.072 (0.5-1)

26.70

0.13 ± 0.059

Drechslera micropa

-

-

26.70

0.2 ± 0.095

13.30

0.1 ± 0.072

33.30

0.2 ± 0.082

Epicoccum purpurascens

20

0.13 ± 0.076 (0.5-1)

-

-

-

-

6.70

0.03 ± 0.033

Fusarium equiseti

26.70

0.5 ± 0.28 (1–4)

40

0.37 ± 0.13 (0.5-1.5)

33.30

0.2 ± 0.08 (0.5-1)

46.70

0.37 ± 0.13 (0.5-1)

Fusarium incarnatum

60

2.5 ± 1.33 (0.5-20)

60

3.43 ± 1.86 (0.5-25.5)

80

4.6 ± 2.5 (0.5-30.5)

80

4.53 ± 2.5 (0.5-36.5)

Fusarium solani

-

-

-

-

6.70

0.03 ± 0.03

13.30

0.1 ± 0.072 (0.5-1)

Fusarium verticillioides

-

-

-

-

33.30

0.23 ± 0.095 (0.5-1)

33.30

0.23 ± 0.095 (0.5-1)

Mucor sp.

6.70

0.03 ± 0.033

-

-

6.70

0.067 ± 0.067

20

0.2 ± 0.10

Myrothecium verrucaria

20

0.17 ± 0.093 (0.5-1)

13.30

0.1 ± 0.072 (0.5-1)

33.30

0.37 ± 0.18 (0.5-2.5)

46.70

0.47 ± 0.2 (0.5-3)

Penicillium sp.

73.30

0.57 ± 0.14 (0.5-2)

66.70

0.7 ± 0.17 (0.5-1.5)

66.70

0.8 ± 0.23 (0.5-2.5)

66.70

1.17 ± 0.36 (0.5-4)

Phoma medicaginis

-

-

33.30

0.23 ± 0.10 (0.5-1.5)

6.70

0.07 ± 0.07

-

-

Rhizoctonia solani

-

-

13.30

0.1 ± 0.072 (0.5-1)

13.30

0.1 ± 0.072 (0.5-1)

13.30

0.13 ± 0.1 (0.5-1.5)

Rhizopus stolonifer

33.30

0.4 ± 0.24 (0.5-3.5)

13.30

0.067 ± 0.045

13.30

0.23 ± 0.2 (0.5-3)

33.30

0.47 ± 0.29 (0.5-4.5)

Sclerotium bataticola

-

-

13.30

0.43 ± 0.40

20

0.13 ± 0.076

-

-

Stachybotrys chartarum

6.70

0.03 ± 0.033

13.30

0.67 ± 0.045

-

-

20

0.13 ± 0.076 (0.5-1)

Stemphylium botryosum

73.30

1.87 ± 0.5 (0.5-5)

80

1.43 ± 0.45 (0.5-5)

60

1.33 ± 0.5 (0.5-6)

53.30

1.37 ± 0.56 (0.5-6.5)

Stemphylium sp.

66.7

0.8 ± 0.19 (0.5-2)

46.7

0.7 ± 0.28 (0.5-4)

40

0.77 ± 0.37 (0.5-5)

26.7

0.43 ± 0.21 (0.5-2.5)

Trichoderma harzianum

33.30

0.6 ± 0.27 (0.5-3)

20

0.17 ± 0.093 (0.5-1)

13.30

0.1 ± 0.072 (0.5-1)

20

0.13 ± 0.076 (0.5-1)

Trichothecium roseum

-

-

-

6.70

0.03 ± 0.033

Ulocladium atrum

46.7

0.33 ± 0.1 (0.5-1)

33.3

0.3 ± 0.14 (0.5-2)

26.7

0.4 ± 0.15 (0.5-2)

26.7

0.23 ± 0.13 (0.5-2)