Fig. 2

Production of recombinant Xyn2 and cell growth of K. lactis GG799 profile. A validation experiment was performed to verify the efficiency of the optimal design medium in which both responses were set at their maximum level using statistical software. The method involves pulling together both first-order models and determining a set of conditions that is a compromise to the stated goals. Both () production of recombinant xylanase and () cell growth of K. lactis GG799 were obtained using a designed medium after screening by Plackett-Burman methods. Each line represents the mean ± standard error (n = 3). The recombinant xylanase production started in the early exponential growth phase and increased to its maximum activity and later decreased when glucose concentrations became a limiting growth factor