Table 2 Plackett-Burman experimental design combinations of factors and levels for recombinant Xyn2 and biomass production using K. lactis GG799
Run no. | Levels | Variables | Responses | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
(NH4)2SO a4 | MgSO∙7H2Oa | Na2SO b4 | FeSO4∙7H2Oa | Casamino acidc | CuSO4∙5H2Ob | ZnSO4∙6H2Oa | MnSO∙4H2Ob | CaCl∙2H2Ob | K2SO b4 | KH2PO a4 | Xylanase activity (U/ml) | Biomass concentration (g/l) | ||
X1 (g/l) | X2 (g/l) | X3 (g/l) | X4 (g/l) | X5 (g/l) | X6 (g/l) | X7 (g/l) | X8 (g/l) | X9 (g/l) | X10 (g/l) | X11 (g/l) | ||||
+ | 15.00 | 3.00 | 0.18 | 0.003 | 5.00 | 0.005 | 0.40 | 0.005 | 0.003 | 3.00 | 8.00 | |||
− | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||
1 | − | + | − | − | − | + | + | + | − | + | + | 40.73 | 4.99 | |
2 | − | − | − | − | − | − | − | − | − | − | − | 40.81 | 4.52 | |
3 | − | − | + | + | + | − | + | + | − | + | − | 39.57 | 4.94 | |
4 | + | − | + | + | − | + | − | − | − | + | + | 45.56 | 4.08 | |
5 | − | − | − | + | + | + | − | + | + | − | + | 40.96 | 5.38 | |
6 | + | + | − | + | − | − | − | + | + | + | − | 19.52 | 5.34 | |
7 | + | + | − | + | + | − | + | − | − | − | + | 19.49 | 5.53 | |
8 | + | + | + | − | + | + | − | + | − | − | − | 36.92 | 5.24 | |
9 | − | + | + | + | − | + | + | − | + | − | − | 43.26 | 4.55 | |
10 | − | + | + | − | + | − | − | − | + | + | + | 20.71 | 5.85 | |
11 | + | − | + | − | − | − | + | + | + | − | + | 41.91 | 4.60 | |
12 | + | − | − | − | + | + | + | − | + | + | − | 23.57 | 5.44 | |