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Table 1 Summary of experimental results obtained in the batch cultures

From: Culture medium of diluted skimmed milk for the production of nisin in batch cultivations

Assay number

Agitation (rpm)

Air flow (L/min)

Beginning of stationary phase (h)

ÎŒmax (h−1)

Xmax (CFU/mL)

P Nisin (mg/L)

Prodx (CFU/mL/h)

ProdNisin(mg/L/h)

Y

kL a (h−1)

1

100

–

14

0.12

5.75.107

12.43

4.11.106

0.89

4.88

6.45.10−3

2

100

0.5

16

0.14

1.45.108

9.95

9.06.106

0.62

2.07

3.61

3

200

–

16

0.12

9.55.107

49.88

5.97.106

3.12

5.64

5.29.10−3

5

200

0.5

16

0.13

2.00.108

15.77

1.25.107

0.99

3.16

5.96

6

200

1.0

16

0.17

1.66.108

17.70

1.04.107

1.11

2.88

9.82

7

200

2.0

16

0.16

1.58.108

17.70

9.91.106

1.11

2.33

4.33

  1. ÎŒmax, Maximum specific growth rate; Xmax, maximum cell concentration; PNisin, maximum nisin concentration; Prodx, cell productivity; ProdNisin, nisin productivity; Y, correlation between nisin activity (log AU/mL) and cell concentration (log CFU/mL); kLa, volumetric oxygen transfer coefficient. All of these parameters were determined at the beginning of stationary phase
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Annals of Microbiology

ISSN: 1869-2044

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