Production of microbial biomass protein from mixed substrates by sequential culture fermentation of Candida utilis and Brevibacterium lactofermentum

Table 2 Kinetics of crude protein production by B. lactofermentum (BL), C. utilis (CU) and BL + CU following growth in CSTR* fed with CSL enriched mixed substrate medium (pH 6.0) at 35°C as in Table 1 but in 75-L fermentor. Values are means ± standard deviation of n = 3 experiments. Values with the same lower case letters are significantly different at P < 0.05

Kinetic parameter	BL	CU	CU + BL	P
Q_CP (g/L h)	1.45. ± 0.04 b	1.56 ± 0.02 b	1.76 ± 0.03 a	<0.0008
Y_CP/S (g/g )	0.45 ± 0.035	0.51 ± 0.02	0.57 ± 0.03	>0.0613
Y_CP/X (g/g cells)	1.1 ± 0.04 b	1.27 ± 0.04 a	1.37 ± 0.05 a	> 0.0018
Q_S (g/L h)	2.52 ± 0.15	2.72 ± 0.25	2.82 ± 0.26	>0.322
Q_X (g/L h)	1.10 ±0.05 b	1.180 ± 0.05 b	1.32 ± 0.04 a	>0.0034
Y_X/S (g/g)	0.45 ± 0.03	0.50 ± 0.04	0.53 ± 0.04	>0.0945

*CSTR run on 80 g reducing sugars/L medium fortified with 30 g/L CSL. Dissolved oxygen level was maintained as described in Materials and methods. Samples were collected periodically and data collected as described earlier

ISSN: 1869-2044