Table 2 Mycelial viability of A. blazei cryopreserved at −70°C with different cryoprotective solutions for 1 and 4 years
From: Viability of Agaricus blazei after long-term cryopreservation
Cryoprotective solutiona | Viability after 1 year (%) | Viability after 4 years (%) | ||
|---|---|---|---|---|
Freezing protocol | Freezing protocol | |||
from 25 to 4 to −70°C | from 25 to −70°C | from 25 to 4 to −70°C | from 25 to −70°C | |
DMSO 1% | 96 | 100 | 80 | 20 |
GLY 6% | 95 | 95 | 73 | 27 |
SAC 15% | 100 | 100 | 100 | 100 |
GLU 4% | 100 | 100 | 100 | 100 |
PEG 8% | 100 | 90 | 46 | 67 |
MEX 5% | 76 | 60 | 60 | 27 |