Table 3 PFGE standardization for the three studied Lactobacillus species
Species | Restriction enzymes | Optimal conditions for electrophoretic separation | Result |
|---|---|---|---|
L. fermentum | SfiI | pulses: 5.0–45 s; 5.0 V/cm; 22 h | Very small restriction fragments below 97 kb |
NotI | pulses: 1.0–40 s; 6.0 V/cm; 22 h | Very small restriction fragments below 97 kb | |
SmaI | pulses: 0.1–6.0 s; 4.5 V/cm; 22 h | Very small restriction fragments below 97 kb | |
SgsI | pulses: 1.0–25 s; 5.5 V/cm; 24 h | Optimal bands distribution on gel | |
L. gasseri | SmaI | pulses: 3.0–13 s; 5.0 V/cm; 22 h | Optimal bands distribution on gel |
L. plantarum | SfiI | pulses: 1.0–20 s; 4.5 V/cm; 26 h | Optimal bands distribution on gel |
NotI | pulses: 1.0–15 s; 6.0 V/cm; 24 h | Small restriction fragments below 145 kb | |
SgsI | pulses: 1.0–25 s; 5.5 V/cm; 24 h | Optimal bands distribution on gel |