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Fig. 1 | Annals of Microbiology

Fig. 1

From: Development of a PCR-RFLP assay for the identification of Lactococcus lactis ssp. lactis and cremoris

Fig. 1

PCR amplification of a fragment of the glutamate decarboxylase (gadB) gene and gadBAseI of Lactococcus lactis ssp. lactis and cremoris. Lane 1–5, 9–10, 12 and 15–16 = test isolates (600 bp), 7 = E. faecalis NCDC 114, 11 = Leuconostoc mesenteroides NCDC 029,13 = B. subtilis NCDC 215, 6 and 14 = L. lactis ssp. lactis NCDC 094, 19 = L. lactis ssp. cremoris ATCC 19297, 20 = L. lactis ssp. cremoris NCDC 086 (560 bp), 8 = 100 bp DNA ladder, 17 & 22 = 50 bp DNA ladder, 18 = 1 kb DNA ladder, 23–27 = gad B-AseI in test isolates 21 = gad B-AseI in L. lactis ssp. cremoris ATCC 19297 and 28 = gad B-AseI in L. lactis ssp. lactis NCDC 094

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