Table 4 PCR-RFLP patterns of a 265 bp fragment encoding the serine protease (htrA) of L. lactis ssp. lactis and cremoris
Strain | PCR product (bp) | AluI | BamHI | HindIII | HinfI | PvuII | SacI | TaqI |
|---|---|---|---|---|---|---|---|---|
L. l. ssp. lactis NCDC 094 | 265 | NFa | NF | 30, 235 | NF | NF | NF | 90, 175 |
L. l. ssp. cremoris ATCC 19297 | 265 | 87,178 | NF | NF | NF | NF | NF | 66, 199 |
L. l. ssp. cremoris NCDC 086 | 265 | 87,178 | NF | NF | NF | NF | NF | 66, 199 |
Test isolates | 265 | NF | NF | 30, 235 | NF | NF | NF | 90, 175 |