Fig. 4

Relationship between 10-fold serially diluted DNA and Ct value (a). A linear range was observed for DNA concentrations from 85 fg μl⁻¹ to 85 ng μl⁻¹. Relationship between the serially diluted P. mirabilis cells and Ct values obtained (b, c). Lines indicate results of regression analysis (triplicate samples). A linear range was obtained from 1.0 × 10¹ CFU ml⁻¹ to 1.0 × 10⁸ CFU ml⁻¹ P. mirabilis cells (b), and a more rigorous linear range was obtained from 1.0 × 10⁴ CFU ml⁻¹ to 1.0 × 10⁸ CFU ml⁻¹ P. mirabilis cells (c)