Quick identification and quantification of Proteus mirabilis by polymerase chain reaction (PCR) assays

Annals of Microbiology

Table 2 Comparison of the PCR-based method for the detection of P. mirabilis

Primers	Gene	Length (bp)	Detection limit	References
ureC1: 5′ -CCGGAACAGAAGTTGTCGCTGGA- 3′	ureC	533	7 × 10³ CFU ml⁻¹	Huang et al. 1999;Takeuchi et al. 1996
ureC2: 5′ - GGGCTCTCCTACCGACTTGATC- 3′	ureC	533	7 × 10³ CFU ml⁻¹	Huang et al. 1999;Takeuchi et al. 1996
MMKAP1: 5′ - ACCTAGTCCCAGAAAAGAACCCTC- 3′	Unknown	3,500	10 fg	Mansy et al. 1999
MMKAP2: 5′ - TGGTGTTAATCACATGCTTGATGG- 3′	Unknown	3,500	10 fg	Mansy et al. 1999
Pr6: 5′ - GGAAACGGTGGCTAATACCGCATAAT- 3′	16S rRNA	101	–	Limanskiĭ et al. 2005
Pr7: 5′ - GCAGCGCTAGGTGAGCCTAATGGG- 3′	16S rRNA	101	–	Limanskiĭ et al. 2005
ureRF1: 5′ - GGTGAGATTTGTATTAATGG - 3′	ureR	225	1.0 × 10¹ CFU ml⁻¹	This study
ureRR1: 5′- ATAATCTGGAAGATGACGAG – 3′	ureR	225	1.0 × 10¹ CFU ml⁻¹	This study

ISSN: 1869-2044