Table 1 Purification of the recombinant CE1 from E.coli JM109 (DE3) containing pET-28a-CE1
From: Cloning, expression and characterization of a novel esterase from Bacillus pumilus
Step | Total protein (mg) | Total activity (U) | Specific activity (U mg−1) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
Crude extract | 18.4 | 66.5 | 3.6 | 1 | 100 |
Nickel affinity chromatography | 7.0 | 40.0 | 5.7 | 1.6 | 60 |