Skip to main content

Table 2 Kinetic parameters for the recombinant CE1 activity on various pNP-esters

From: Cloning, expression and characterization of a novel esterase from Bacillus pumilus

pNP-esters

K m (mM)

V max (U mg−1)

k cat (s−1)

k cat/ K m (s−1·mM−1)

Acetate (C2)

0.33 ± 0.02

8.28 ± 0.11

4.07 ± 0.05

12.37 ± 0.61

Butyrate (C4)

0.37 ± 0.01

5.15 ± 0.13

2.53 ± 0.06

6.82 ± 0.32

Caprylate (C8)

3.89 ± 0.57

2.95 ± 0.26

1.45 ± 0.13

0.37 ± 0.02

  1. The kinetic parameters were determined at substrate concentrations ranging from 0.1 to 2.0 mM for pNP acetate, from 0.1 to 1.0 mM for pNP butyrate, and from 0.2 to 2.4 mM for pNP caprylate. Data are reported as means ± standard deviation of triplicates.
Back to article page

Annals of Microbiology

ISSN: 1869-2044

Contact us