Isolation and characterization of Listeria monocytogenes from tropical seafood of Kerala, India

Annals of Microbiology

Table 2 Primers used in this study

Primer pair and sequences (5′–3′)	Specificity	References	Product size (bp)
UI (CAGCMGCCGCGGTAATWC) and LII (CTCCATAAAGGTGACCCT)	Listeria genus	Border et al. 1990	938
LL5 (AACCTATCCAGGTGCTC) and LL6 (CTGTAAGCCATTTCGTC)	L. monocytogenes	Herman et al. 1995	267
lin0464F (CGCATTTATCGCCAAAACTC) and lin0464R (TCGTGACATAGACGCGATTG)	L. innocua	Liu et al. 2003	749
Forward (CTGCTTGAGCGTTCATGTCTCATCCCCC) and Reverse (CATGGGTTTCACTCTCCTTCTAC)	plcA gene of L. monocytogenes	Notermans et al. 1991; Rawool et al. 2007	1,484
Forward (CTGTTGGAGCTCTTCTTGGTGAAGCAATCG) and Reverse (AGCAACCTCGGTACCATATACTAACTC)	prfA gene of L. monocytogenes	Notermans et al. 1991; Rawool et al. 2007	1,060
Forward (GCAGTTGCAAGCGCTTGGAGTGAA) and Reverse (GCAACGTATCCTCCAGAGTGATCG)	hlyA gene of L. monocytogenes	Paziak-Domanska et al. 1999; Rawool et al. 2007	456
Forward (ACAAGCTGCACCTGTTGCAG) and Reverse (TGACAGCGTGTGTAGTAGCA)	iap gene of L. monocytogenes	Furrer et al. 1991; Rawool et al. 2007	131
Forward (CGCCGCGGAAATTAAAAAAAGA) and Reverse (ACGAAGGAACCGGGCTGCTAG)	actA gene of L. monocytogenes	Rawool et al. 2007	839
Forward (TGGTAATAGCTTTTCAGGCTCA) and Reverse (CTTTAAGGCGCGGAAGTAAAG)	mpl gene of L. monocytogenes	Designed for this study	1,376

ISSN: 1869-2044