Table 2 Box–Behnken design for optimizing the significant variables for biomass and PHB production
Run order | Inoculum size (ml) | (NH4)2SO4 content (%) | pH | Biomass (g/g PUF) | PHB (g/g PUF) |
|---|---|---|---|---|---|
1 | 6.5 | 1.7 | 9.5 | 0.4 | 0.169 |
2 | 6.5 | 0.4 | 8.0 | 0.19 | 0.05 |
3 | 6.5 | 3.0 | 11.0 | 0.056 | 0.003 |
4 | 6.5 | 3.0 | 8.0 | 0.5 | 0.06 |
5 | 5.0 | 0.4 | 9.5 | 0.1 | 0.04 |
6 | 6.5 | 1.7 | 9.5 | 0.38 | 0.168 |
7 | 6.5 | 1.7 | 9.5 | 0.376 | 0.165 |
8 | 5.0 | 1.7 | 11.0 | 0.028 | 0.002 |
9 | 8.0 | 3.0 | 9.5 | 0.298 | 0.02 |
10 | 8.0 | 1.7 | 11.0 | 0.05 | 0.002 |
11 | 8.0 | 0.4 | 9.5 | 0.089 | 0.005 |
12 | 8.0 | 1.7 | 8.0 | 0.40 | 0.019 |
13 | 6.5 | 0.4 | 11.0 | 0.02 | 0.003 |
14 | 5.0 | 1.7 | 8.0 | 0.38 | 0.02 |
15 | 5.0 | 3.0 | 9.5 | 0.2 | 0.005 |