Fig. 2

Integration of the zwf gene into the lacZ′ gene of the pGEM-T Easy vector. The zwf fragment is shown in italics and is indicated with a horizontal bar below. The 5′ of the fragment EcoRI and SacI recognition sites were added. Also, a G was added upstream the ATG start position of the zwf gene in order to keep the zwf codons in frame with lacZ′. From such a construct, the Zwf polypeptide would be synthesized with a 12 amino acid tag. The start positions of both the lacZ′ and the zwf genes are indicated with vertical arrows