Fig. 3

Analysis of the pGEM-T Easy-zwf construct. a The construct carrying the zwf gene in the same orientation as lacZ′ gene of pGEM-T vector. Two SacI sites are present, one 5′ of the lacZ′ gene and the other 5′ of the zwf gene. The sites are 57 bp apart from each other. In case of the reverse orientation, the distance between the sites must be 1,654 bp. b Restriction fragment length analyses of the two recombinant plasmids. Lane 1 is SacI cut fragments of an isolated plasmid with two fragments, one of which is 1,645 bp, indicating a reverse orientation of the zwf. Lane 2 is another SacI cut plasmid with a 4,575 bp fragment. The second 55 bp fragment could not be seen because of running out of the gel. This result shows that the plasmid shown in lane 2 is carrying the zwf gene in the same orientation with lacZ′. M is DNA size marker Lamda DNA/HindIII