Table 1 The plasmids and E. coli strains used in this study

 pGEM-T easy

Amp^r, lacZ′ ⁺

Marcus et al. 1996

 pNUT1

pUC19 carrying a 2.9 kb zwf fragment of Synechococcus sp. PCC 7942, Amp^r

Scanlan et al. 1992

 pSG162

pGEM-T carrying 1.6 kb zwf fragment in MCS of lacZ′ gene in same orientation, Amp^r

In this study

 E. coli DH5α

Standard E. coli strain, zwf ⁺

Grant et al. 1990

 E. coli JM109

Standard E. coli strain, zwf ⁺

Yanisch-Peron et al. 1985

 E. coli DF214

Δzwf and pgi ^- strain, no glucose utilisation

Vinapol et al. 1975

 SZJ29

E. coli JM109 carrying pNUT1

In this study

 SZD29

E. coli DF214 carrying pNUT1

In this study

 AY162

E. coli DF214 carrying pSG162

In this study