Fig. 2

Physical map and genetic organization of pBMB2062. The outer circle represents the small plasmid pBMB2062, and its ORFs are represented as solid arrows showing the direction of transcription with the length of the arrow reflecting the relative lengths of the genes. The nick sites and promoters are indicated with arrows. P1 (nt at positions 1,273–1,292 on pBMB2062) and P2 (nt at positions 1,520–1,539 on pBMB2062) are the primers used to generate a fragment containing a deletion mutant of the ORF2 gene that was used to construct plasmid BMB0402. P3 (nt at positions 1,025–1,044 on pBMB2062) and P4 (nt at positions 216–235 on pBMB2062) are the primers used to generate a fragment containing a deletion mutant of the ORF1 gene that was used to construct plasmid BMB0403. P5 (nt at positions 1,085–1,104 on pBMB2062) and P6 (nt at positions 235–254 on pBMB2062) are the primers used to generate the ORF1 fragment. To simplify the description, pBMB2062 starts at the same origin as p2055 and p2058 in Figs. 2 and 3