Table 1 Distribution of pBMB2062 in Bacillus thuringiensis strains covered all the serotypes plus one B. cereus strain by using dot-blots method with the ORF1-pBMB2062 fragment as probe

Strains showed positive signal

Bacillus thuringiensis 4B1 (H2), 4D1 (H3abc), HD-1 (H3abc), YBT-1406 (H3abc), YBT-1463(H3abc), YBT-1520 (H3abc), 4F1 (H4ac), CT-155 (H4ac), CT-256 (H4ac), 4G1 (H5ab), 4H1 (H5ac), YBT-1416 (H7), 4AA1 (H8ab), 4Z1 (H8ac), 4L1 (H9), 4BF1 (H10ac), 4O1 (H12), 4Y1 (H19), 4AM1 (H20ab), 4X1 (H21), 4AL1 (H25), 4AG1 (H26), 4AC1 (H27), 4CF1 (H28ac), 4AE1 (H29), 4AD1 (H31), 4AF1 (H32), 4AK1 (H33), YBT-833 (H33), 4AW1 (H37), 4BD1 (H40), 4AR1 (H42), 4BJ1 (H47), 4BL1 (H49), 4BS1 (H55), 4BT1 (H56), 4CB1 (H64), T68 (H68), T69 (H69), CT-43 (no flagellum), YBT-031 (unknown serotype), YBT-9603 (unknown serotype), MGR (unknown serotype) and B. cereus strain UW85

Strains showed no signal

Bacillus thuringiensis 4A1 (H1), HD-a2 (H1), BMB171 (H3abc, plasmid-free mutant), 4C1 (H3ac), 4AP1 (H3ade), 4E1 (H4ab), 4I1 (H6), 4J1 (H7), 4K1 (H8ab), YBT-1765 (H8ab), 4AZ1 (H8bd), 4M1 (H10ab), YBT-1532 (H10ab), 4N1 (H11ab), 4U1 (H11ac), 4P1 (H13), 4Q1 (H14), YBT-007 (H14), YBT-008 (H14), IPS78/11 (H14), 4R1 (H15), 4S2 (H16), 4V1 (H17), 4W1 (H18ab), 4CA1 (H18ac), 4BA1 (H20ac), 4AN1 (H22), 4AT1 (H23), 4BE1 (H24ab), 4AX1 (H24ac), 4AJ1 (H28ab), T30 (H30), 4AD1 (H31), 4AH1 (H34), 4AQ1 (H35), 4AV1 (H36), 4AY1 (H39), 4BB1 (H41), 4BC1 (H43), 4AU1 (H44), 4BG1 (H45), 4BH1 (H46), 4BK1 (H48), 4BM1 (H50), 4BN1 (H51), 4BP1 (H52), 4BQ1 (H53), 4BR1 (H54), 4BU1 (H57), 4BV1 (H58), 4BW1 (H59), 4BX1 (H60), 4BY1 (H61), 4BZ1 (H62), T63 (H63), 4CC1 (H65), 4CD1 (H66), 4CE1 (H67), T70 (H70), T71 (H71), 4T1 (no flagellum), YBT-1514 (unknown serotype), BMB999-7 (unknown serotype) and YBT-1345 (unknown serotype).