Fig. 2From: ComCED signal loop precisely regulates nlmC expression in Streptococcus mutans Electrophoretic mobility shift assay of ComE with 4 selected DNA fragments. a, b, c, d: EMSAs of ComE with PnlmAB, PnlmC, PnlmC314, or PnlmC419, respectively. M: DNA ladder (Invitrogen 10068–013). Lanes 1–4: ComE concentration gradient of 0, 326, 652, and 1,305 ng per 20 μL volume, respectively. FD: Free DNA. b1, b2, b3: main retarded DNABack to article page