From: Production and characterization of laccase from Pleurotus ferulae in submerged fermentation
Purification step | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Yield (%) | Purification fold |
---|---|---|---|---|---|
Culture supernatant | 2,171 | 99.68 | 21.78 | 100 | 1 |
(NH4)2SO4 precipitation | 1,769 | 65.01 | 27.21 | 81.48 | 1.25 |
Mono Q | 881.6 | 20.91 | 42.16 | 40.60 | 1.94 |
Superdex 75 | 584.2 | 5.53 | 105.6 | 26.91 | 4.85 |