Table 2 Comparison of the enzymatic properties of different laccase isolated from Pleurotus spp.
From: Production and characterization of laccase from Pleurotus ferulae in submerged fermentation
Source of laccase | Optimal temperature (°C) | Optimal pH | Molecular mass (kDa) | t 1/2 | K m (mM) | K cat/K m | Reference |
|---|---|---|---|---|---|---|---|
Pleurotus ostreatus PO XA3a | 35 | ABTS = 3.6 | 85 | 6 h | ABTS = 7.0 × 10−2 | ABTS = 6.3 × 107 (mM  min)−1 | Palmieri et al. 2003 |
Syringaldazine = 6.2 | Syringaldazine = 3.6 × 10−2 | Syringaldazine = 4.7 × 106 (mM  min)−1 | |||||
DMP = 5.5 | DMP = 14 | DMP = 1.0 × 105 (mM  min)−1 | |||||
Pleurotus ostreatus PO XA3b | 35 | ABTS = 3.6 | 83 | 14 h | ABTS = 7.4 × 10−2 | ABTS = 1.3 × 108 (mM min)−1 | |
Syringaldazine = 6.2 | Syringaldazine = 7.9 × 10−2 | Syringaldazine = 8.9 × 106 (mM min)−1 | |||||
DMP = 5.5 | DMP = 8.8 | DMP = 1.4 × 105 (mM min)−1 | |||||
Pleurotus florida | 50 | o-Bianisidine = 5 | 77 | o-Bianisidine = 0.056 s | o-Bianisidine = 0.13 | o-Bianisidine = 6.32 × 107 (mM s)−1 | Das et al. 2000 |
Guaiacol = 5 | Guaiacol = 92.4 s | Guaiacol = 30.0 | Guaiacol = 1.92 × 103 (mM s)−1 | ||||
Pleurotus sajor-caju | 40 | ABTS = 4.5–5.0 | 61 | n.d.a | ABTS = 5.6 × 10−2 | ABTS = 9.2 × 105(M s)−1 | Murugesan et al. 2006 |
Syringaldazine = 4.2 × 10−2 | Syringaldazine = 8.7 × 105 (M s)−1 | ||||||
Pleurotus eryngii | 70–80 | ABTS = 3–5 | 34 | n.d. | n.d. | n.d. | Wang and Ng 2006 |
Pleurotus ostreatus D1 | n.d. | Syringaldazine = 7.0 | 64 | n.d. | Syringaldazine = 0.0087 | n.d. | Pozdnyakova et al. 2006 |
Pyrocatechol = 8.0 | Pyrocatechol = 3.65 | ||||||
ABTS = 4.0 | ABTS = 0.11 | ||||||
DMP = 4.0 | DMP = 0.43 | ||||||
Pleurotus pulmonarius | 50 | Syringaldazine = 6.2–6.5 | 46 | n.d. | Syringaldazine = 1.2 × 10−2 | Syringaldazine = 54.50 (mM s)−1 | De Souza and Peralta 2003 |
ABTS = 4.0–5.5 | ABTS = 2.1 × 10−1 | ABTS = 7.23(mM s)−1 | |||||
Guaiacol = 6.0–8.0 | Guaiacol = 5.5 × 10−1 | Guaiacol = 0.56(mM s)−1 | |||||
Pleurotus ostreatus strain 10969 | 4.0 | ABTS = 50 | 40 | n.d. | ABTS = 0.31 | n.d. | Liu et al. 2009 |
Pleurotus florida | 50 | n.d. | 70 | o-dianisidine = 0.012 s | o-dianisidine = 0.045 | o-dianisidine = 4.13 × 107 (mM s)−1 | Das et al. 2001 |
Guaiacol = 1.47 s | Guaiacol = 2.81 | Guaiacol = 3.31 × 105 (mM s)−1 | |||||
Pleurotus ostreatus | n.d. | n.d. | 55 | n.d. | ABTS = 15 × 10−2 | n.d. | Lettera et al. 2010 |
DMP = 27 × 10−2 | |||||||
Syringaldazine = 5.5 × 10−2 | |||||||
Pleurotus ostreatus | n.d. | n.d. | 71 | n.d. | o-dianisidine = 1.72 × 10−2 | n.d. | Freixo et al. 2012 |
Guaiacol = 4 | |||||||
ABTS = 5.52 × 10−2 | |||||||
Pleurotus cornucopiae | 40 | 4 | 66 | n.d. | n.d. | n.d. | Wong et al. 2010 |
P. ferulae JM30X | 50 | 3 | 66 | 50 °C = 7.59 h | ABTS = 0.19 | ABTS = 2.73 × 106(mM s)−1 | This work |
DMP = 3.77 | DMP = 4.54 × 104 (mM s)−1 | ||||||
Veratryl alcohol = 70.21 | Veratryl alcohol = 6.85 × 104 (mM s)−1 | ||||||
Guaiacol = 22.24 | Guaiacol = 1.87 × 103 (mM s)−1 | ||||||
Tyrosine = 6.87 | Tyrosine = 1.21 × 105 (mM s)−1 |