Table 2 Restriction enzymes and their respective sites for the fliC, 16S rRNA and fimH genes
Restriction enzyme | Recognition site | Position of restriction in fliC | aPosition of restriction in 16S rRNA gene | Position of restriction in fimH |
|---|---|---|---|---|
EcoRI | G…AATTC | – | 674 | – |
SmaI | CCC…GGG | – | 614 | – |
EcoRV | GAT…ATC | 472 | – | 103, 640 |
MluI | A…CGCGT | – | – | 534 |
HincII | GTPy…PuAC | – | – | 388 |
ClaI | AT…CGAT | 468, 474 | – | 755 |
HaeIII | GG…CC | 384,1424 | 46,213,417 | – |
ScaI | AGT…ACT | 604 | 736,898,932,1141 | – |