Comparison of bacteriocins production from Enterococcus faecium strains in cheese whey and optimised commercial MRS medium

Schirru, Stefano; Favaro, Lorenzo; Mangia, Nicoletta Pasqualina; Basaglia, Marina; Casella, Sergio; Comunian, Roberta; Fancello, Francesco; de Melo Franco, Bernadette Dora Gombossy; de Souza Oliveira, Ricardo Pinheiro; Todorov, Svetoslav Dimitrov

doi:10.1007/s13213-013-0667-0

Table 3 Influence of each component on the production of bacteriocins SD1, SD2, SD3 and SD4 in modified MRS broths incubated at 30 °C for 24 h

From: Comparison of bacteriocins production from Enterococcus faecium strains in cheese whey and optimised commercial MRS medium

Component^a	Concentration (g/L)	Bacteriocin activity (AU/mL)
Component^a	Concentration (g/L)	SD1	SD2	SD3	SD4
Nitrogen sources
Control (peptone + meat extract + yeast extract)	10 + 8 + 4	25,600	25,600	1,600	800
Tryptone	20	25,600	6,400	1,600	800
Meat extract	20	6,400	6,400	400	400
Yeast extract	20	6,400	1,600	400	400
Tryptone + meat extract	12.5 + 7.5	12,800	3,200	200	200
Tryptone + yeast extract	12.5 + 7.5	6,400	6,400	200	200
Meat extract + yeast extract	10 + 10	25,600	3,200	400	400
Tryptone + meat extract + yeast extract	10 + 5 + 5	25,600	25,600	1,600	800
Sugar sources
Control (glucose)	20	25,600	25,600	1,600	800
Glucose	5	12,800	3,200	200	200
Glucose	10	12,800	6,400	200	200
Glucose	30	25,600	6,400	400	200
Glucose	50	1,600	800	200	0
Lactose	5	25,600	51,200	800	400
Lactose	10	51,200	51,200	3,200	400
Lactose	20	25,600	51,200	1,600	200
Lactose	30	12,800	51,200	400	200
Lactose	50	6,400	25,600	200	200
d-Mannitol	20	NG^b	NG	NG	NG
Mannose	20	25,600	51,200	1,600	800
Rhamnose	20	NG	6,400	NG	0
Inorganic salts
Control (di-potassium phosphate + magnesium sulphate + manganese sulphate + tri-ammonium citrate + sodium acetate)	2 + 0.1 + 0.05 + 2 + 5	25,600	25,600	1,600	800
Di-potassium phosphate + mono-potassium phosphate	2 + 2	3,200	3,200	0	0
Di-potassium hydrogen phosphate	0	NG	NG	200	200
Di-potassium hydrogen phosphate	5	12,800	1,600	400	200
Di-potassium hydrogen phosphate	10	25,600	800	800	200
Mono-potassium phosphate	0	NG	NG	200	200
Mono-potassium phosphate	2	NG	NG	NG	NG
Mono-potassium phosphate	5	NG	NG	200	NG
Mono-potassium phosphate	10	NG	NG	0	0
Magnesium sulphate	0	6,400	400	200	200
Manganese sulphate	0	3,200	6,400	200	400
Tri-ammonium citrate	0	12,800	6,400	400	400
Tri-ammonium citrate	4	1,600	12,800	0	0
Sodium acetate	0	3,200	1,600	400	400
Sodium acetate	10	1,600	1,600	200	200
Other components
Control (Tween 80)	1	25,600	25,600	1,600	800
Tween 80	2	3,200	6,400	200	200
Tween 80	5	3,200	6,400	200	200
Glycerol	0	25,600	25,600	1,600	800
Glycerol	2	6,400	6,400	400	400
Glycerol	5	3,200	6,400	400	400
Glycerol	8	3,200	12,800	400	400
Glycerol	10	6,400	12,800	400	200
Glycerol	20	6,400	6,400	200	200

^aControl means normal components of MRS broth
^bNo growth

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ISSN: 1869-2044

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