Fig. 2
From: Enhancing expression level of an acidophilic β-mannanase in Pichia pastoris by double vector system
Effects of initial pH value and methanol concentration on expression level of the reAuMan5A. a The GSKZαM4-2 was inoculated into BMMY media with initial pH values ranging from 5.0 to 8.0, and induced using the standard protocol. The statistical comparison between pH 7.0 and original pH 6.0 designated by standard protocol was significant at P < 0.01. b Inoculated into BMMY media (pH 7.0), the GSKZαM4-2 was induced by adding methanol concentrations from 0.5 to 3.0 %. The statistical comparison between the optimized and original methanol concentrations was significant at P < 0.05