Fig. 3From: Enhancing expression level of an acidophilic β-mannanase in Pichia pastoris by double vector systemEffects of induction temperature and period on expression level of the reAuMan5A. The GSKZαM4-2 was induced in a BMMY medium (pH 7.0) by adding methanol of 1.5 % at 24-h intervals until 144 h at three temperatures of 28, 30 and 32 °C, respectivelyBack to article page