Enhancing expression level of an acidophilic β-mannanase in Pichia pastoris by double vector system

Annals of Microbiology

Table 1 Screening of the P. pastoris transformants for multiple copies of the Auman5A

G418 (mg/ml)	Representative strain	Enzyme activity (U/ml)^a	Zeocin (μg/ml)	Representative strains	Enzyme activity (U/ml)^a
1.0	GSKM1-2	36.7 ± 1.3	100	GSKZαM1-3	63.1 ± 1.1^b
	GSKM1-5	39.0 ± 1.6		GSKZαM1-6	60.5 ± 0.8^b
	GSKM1-7	34.9 ± 1.3		GSKZαM1-7	65.2 ± 1.4^b
2.0	GSKM2-1	40.2 ± 1.2^c	200	GSKZαM2-1	69.4 ± 1.6^b
	GSKM2-3	49.0 ± 1.3^b		GSKZαM2-4	70.8 ± 1.1^b
	GSKM2-6	37.6 ± 1.1		GSKZαM2-9	72.3 ± 1.3^b
4.0	GSKM4-3	49.7 ± 1.4^b	400	GSKZαM4-2	78.1 ± 1.2 ^b
	GSKM4-5	39.2 ± 1.6^c		GSKZαM4-5	73.4 ± 1.5^b
	GSKM4-8	54.6 ± 1.4 ^b		GSKZαM4-7	71.8 ± 1.6^b

Strains showing the highest activity for G418 or Zeocin are highlighted in bold
^aAll data were obtained from three independent experiments and expressed as the mean ± SD
^bThe statistical comparison of β-mannanase activities between the GSKM1-2 and other strains displayed significant differences at P < 0.01
^cThe statistical comparison displayed significant differences at P < 0.05

ISSN: 1869-2044