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Table 2 Factors and level arranged by the L9(34) of ‘orthogonal design’

From: Enhancing expression level of an acidophilic β-mannanase in Pichia pastoris by double vector system

Level b

Factors a

Methanol concentration (A)

Induction temperature (B)

Initial pH value (C)

Induction period (D)

1

1.0 %

28 °C

6.5

96 h

2

1.5 %

30 °C

7.0

108 h

3

2.0 %

32 °C

7.5

120 h

  1. a A, B, C, and D represent four factors of methanol concentration, induction temperature, initial pH value, and induction period, respectively
  2. b 1, 2, and 3 represent three levels of each factor, respectively
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Annals of Microbiology

ISSN: 1869-2044

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