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Table 3 The array and experimental results by the L9(34) of ‘orthogonal design’

From: Enhancing expression level of an acidophilic β-mannanase in Pichia pastoris by double vector system

Number

A

B

C

D

reAuMan5A activity (U/ml)

1

1

1

1

1

71.12

2

1

2

2

2

62.78

3

1

3

3

3

40.82

4

2

1

2

3

131.04

5

2

2

3

1

35.85

6

2

3

1

2

132.92

7

3

1

3

2

20.41

8

3

2

1

3

138.33

9

3

3

2

1

70.90

  1. The arrays of levels (1, 2, and 3) in columns (A, B, C, and D) were arranged.
  2. Each of 9 experiments was conducted in triplicate