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Table 4 The analytical results by the L9(34) of ‘orthogonal design’

From: Enhancing expression level of an acidophilic β-mannanase in Pichia pastoris by double vector system

Level

A

B

C

D

K1 = total level 1

174.72

222.57

342.37

177.87

K2 = total level 2

299.81

236.96

264.71

216.10

K3 = total level 3

229.64

244.64

97.09

310.20

k1 = K1/3

58.24

74.12

114.13

59.29

k2 = K2/3

99.93

78.99

88.24

72.03

k3 = K3/3

76.55

81.55

32.37

103.40

R = kmax − kmin

41.69

7.43

81.76

44.11

Affecting order

C > D > A > B

Optimal combination

C1D3A2B3