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Table 1 Total particles, prokaryotes and virus-like particles counts on 0.2- and 0.02-μm pore size membranes by epifluorescence microscopy

From: Proportion of prokaryotes enumerated as viruses by epifluorescence microscopy

 

Sampling station

I2

I6

Pore size membranes

Particles type

Number (average ± SD L−1)

%

Number (average ± SD L−1)

%

0.2 μm

Total (DAPI stain)

4.6 ± 0.3 × 109

100

3.9 ± 0.2 × 109

100

Eubacteria (FISH Eub338 probe stain)

3.2 ± 0.3 × 109

70

2.4 ± 0.4 × 109

61

Archaea (FISH Arc915 probe stain)

3.0 ± 0.1 × 108

7

3.9 ± 0.04 × 108

10

Non-probe labelled (DAPI stain-probes stain)

1.1 ± 0.2 × 109

23

1.2 ± 0.2 × 109

29

0.02 μm

Total (DAPI stain)

1.2 ± 0.06 × 1010

100

1.0 ± 0.09 × 1010

100

Eubacteria (FISH Eub338 probe stain)

2.6 ± 0.6 × 109

22

7.5 ± 0.2 × 109

7

Archaea (FISH Arc915 probe stain)

7.8 ± 0.3 × 108

6

6.1 ± 0.17 × 108

6

Viruses (DAPI stain-probes stain)

8.5 ± 0.6 × 109

72

9.0 ± 0.8 × 109

87

  1. Values represent the mean of three sub-samples. For each sub-sample, three replicates were counted