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Table 2 Gas chromatography–mass spectrometry analysis of volatile compounds of naturally fermented and inoculated fermented Chinese sauerkraut

From: Reduced nitrite and biogenic amine concentrations and improved flavor components of Chinese sauerkraut via co-culture of Lactobacillus plantarum and Zygosaccharomyces rouxii

Retention time (min)

Volatile compound

Concentrationa (mg/l)

Cabbage

Radish

Spontaneous fermentation (SF)

Inoculated fermentation (IF)

Spontaneous fermentation (SF)

Inoculated fermentation (IF)

Acids (22)

 5.07

2-Methyl- propanoic acid

NDb

ND

0.19

0.79*

 7.47

Butanoic acid

5.27

14.32*

0.25

6.56*

 8.47

3-Methyl-butanoic acid

0.30

0.35

0.56

0.51

 8.74

2- menthyl-butanoic acid

0.04

0.36*

0.55

1.37*

 9.99

Pentanoic acid

0.55

0.59

0.13

0.40*

 14.5

Hexanoic acid

0.05

4.71*

ND

2.66*

 16.27

Heptanoic acid

0.33

0.14

0.26

1.04*

 16.73

Furoic acid

0.89

0.90

TR

2.39*

 21.59

Phenylacetic acid

5.45

13.14*

0.38

1.26*

 21.76

Pelargonic acid

3.44

10.18*

14.83

40.46*

 23.8

Phenylpropionic acid

0.33

0.50

0.23

0.82*

 24.28

Capric acid

0.70

0.52

1.38

1.34

 29.09

Dodecanoic acid

2.47

2.91

0.21

1.71*

 33.38

3-Methoxy-4-hyd roxy- phenylpropionic acid

0.60

0.95

0.27

1.06*

 33.54

Myristic acid

8.06

16.49*

0.48

3.09*

 35.6

Pentadecanoic acid

0.94

1.09

0.39

3.38*

 37.24

9-Hexadecylenic acid

3.12

7.83*

0.54

6.13*

 37.85

Hexadecanoic acid

4.13

37.21*

40.23

92.78*

 40.17

15-Methyl- heptadecylic acid

0.28

0.52

0.22

0.74*

 42.9

Linolenic acid

2.37

10.88*

1.17

7.27*

 43.14

Oleic acid

1.38

6.32*

3.35

21.41*

 43.81

Stearic acid

2.96

8.29*

27.98

48.24

Alcohols (4)

 3.46

3-Methyl-1-butanol

0.21

2.00*

0.79

15.73*

 5.22

2,3-Butanediol

ND

ND

0.76

1.96*

 8.01

1-Hexanol

0.01

0.08

0.01

0.03

 12.34

1,2,3-Butantriol

0.01

0.05

ND

0.18*

Esters (20)

 9.95

Methyl hexanoate

0.21

0.28

0.19

0.15

 36.75

Methyl palmitate

2.12

6.42*

2.65

24.62*

 5.11

Ethyl butyrate

4.71

4.07

4.08

6.04

 5.69

Ethyl lactate

0.73

0.59

0.17

0.28

 9.01

Ethyl valerate

0.49

0.49

0.33

0.35

 9.95

Ethyl hexanoate

3.57

7.82*

4.13

5.35

 14.82

2-Hydroxy-4-methyl-ethyl valerate

0.01

0.02

0.01

0.31*

 16.14

Ethyl heptylate

0.21

0.32

0.02

0.58*

 18.86

Diethyl succinate

0.01

0.01

0.01

0.12

 19.2

Ethyl caprylate

1.32

2.71*

1.18

1.36

 22.03

Ethyl pelargonate

1.12

2.56*

0.01

3.15*

 24.47

Hexyl hexanoate

2.02

4.48*

4.18

7.46

 24.7

Ethyl caprate

2.13

1.36

0.24

0.25

 34.03

Ethyl myristate

0.01

0.22*

0.02

0.24*

 35.49

Ethyl pentadecanoate

0.21

0.26

11.67

22.78*

 38.21

Ethyl hexadecanoate

14.22

28.30*

15.05

33.61*

 43.32

Ethyl linoleate

0.08

4.82*

ND

5.72*

 43.59

Ethyl oleate

ND

3.48*

ND

5.37*

 44.61

Ethyl octadecanoate

0.02

0.08

0.18

0.18

Phenols (9)

 8.55

Ortho-xylene

0.01

ND

0.09

0.17

 11.7

Benzaldehyde

0.19

0.11

0.05

1.27*

 14.49

Phenylcarbinol

0.01

0.01

0.01

0.13

 16.97

Phenylethyl alcohol

0.04

0.50*

0.02

2.17*

 18.62

Ethyl benzenecarboxylate

0.14

0.11

0.19

0.25

 20.75

Ethyl phenylacetate

0.25

5.70*

0.25

4.54*

 30.9

Phenyl ethyl butyrate

0.02

0.07

0.05

0.20*

 32.03

6-Ethyl-3-octyl ester- phthalic acid

0.04

0.10

21.36

38.08

Pyrazines (1)

 15.93

Tetramethylpyrazine

0.01

0.07

0.01

0.06

Furans (4)

 6.70

3-Fur(fur)aldehyde

0.14

0.14

ND

0.08

 7.69

2- Furfuralcohol

0.23

0.26

0.05

0.42*

 31.81

5-Methyl −2- carboxylic acid furan formaldehyde

0.06

0.11

0.27

0.50

 34.41

5-Methyl-2-furan formaldehyde

0.01

0.02

0.80

0.65

  1. *Significant difference between SF and IF at p < 0.05 by the Student’s t test
  2. aThe concentration of each compound was calculated on the basis of the ratio of peak area of the respective compounds and that of the internal standard (2-octanol; Sigma-Aldrich, St. Louis, MO). Three individual fermentations were performed, and the data were the mean value of triplicate measurements
  3. bND, Not determined or peak area of <0.05 %