Table 2 RAPD analysis of wild and mutant strains of Lactobacillus brevis NM101-1 using three primers (RP, R5, and M13) under various doses of gamma rays
Marker | Wild | LM-1 | LM-2 | LM-3 | LM-4 | |||||
|---|---|---|---|---|---|---|---|---|---|---|
MWa (bp) | MWa (bp) | Ab | MWa (bp) | Ab | MWa (bp) | Ab | MWa (bp) | Ab | MWa (bp) | Ab |
RP | ||||||||||
1500 | – | – | – | – | – | – | – | – | – | – |
900 | – | – | – | – | – | – | – | – | – | – |
800 | – | – | – | – | – | – | – | – | – | – |
– | – | – | – | – | – | – | 706 | 5.19 | – | – |
600 | – | – | 592 | 3.68 | – | – | 600 | 3.36 | – | – |
– | – | – | 525 | 6.07 | 525 | 9.08 | 533 | 5.26 | – | – |
– | – | – | 467 | 3.2 | – | – | 467 | 4.4 | – | – |
– | – | – | – | – | 442 | 8.79 | 433 | 5.48 | 425 | 4.41 |
400 | – | – | 394 | 12 | 389 | 7.76 | 383 | 8.85 | 389 | 6.71 |
– | – | – | 350 | 5.64 | – | – | 350 | 4.11 | – | – |
300 | – | – | 317 | 4.78 | 322 | 10.7 | 322 | 9.97 | 322 | 11.1 |
– | 255 | 10.9 | 260 | 8.94 | 260 | 8.01 | 260 | 11.4 | 260 | 12.4 |
200 | 185 | 17 | 188 | 10.2 | 191 | 7.35 | 191 | 4.17 | 191 | 20.2 |
– | – | – | – | – | – | 174 | 4.21 | – | – | |
100 | – | – | – | – | – | – | – | – | – | – |
Total | 2 | 8 | 6 | 11 | 5 | |||||
R5 | ||||||||||
1500 | – | – | – | – | – | – | – | – | – | – |
– | 1149 | 12.4 | – | – | – | – | – | – | – | – |
900 | 895 | 13.6 | – | – | – | – | – | – | – | – |
– | – | – | – | – | – | – | 843 | 6.06 | – | – |
800 | 777 | 14 | 766 | 11.7 | 766 | 12.1 | 771 | 20.7 | 783 | 15.9 |
– | – | – | – | – | – | – | 709 | 5.85 | – | – |
– | – | – | – | – | – | – | 657 | 5.67 | – | – |
600 | – | – | – | – | – | – | – | – | 567 | 17.7 |
– | 533 | 9.57 | – | – | 527 | 9.85 | 533 | 14 | – | – |
400 | 400 | 21.2 | 395 | 20.5 | 395 | 25.4 | 393 | 12.7 | 393 | 11.2 |
300 | – | – | – | – | 317 | 6.44 | 315 | 4.39 | 317 | 8.94 |
200 | – | – | – | – | – | – | – | – | – | – |
100 | – | – | – | – | – | – | – | – | – | – |
Total | 5 | 2 | 4 | 7 | 4 | |||||
M13 | ||||||||||
1500 | – | – | – | – | – | – | – | – | – | – |
900 | 900 | 11 | 919 | 7.03 | 919 | 16.9 | 900 | 16.2 | 900 | 23.4 |
800 | – | – | – | – | – | – | – | – | – | – |
600 | 600 | 22.7 | 667 | 20.7 | 667 | 22.3 | 600 | 21.6 | 633 | 23.5 |
400 | – | – | – | – | 392 | 4.96 | 385 | 3.18 | 400 | 3.7 |
300 | – | – | – | – | 300 | 4.95 | 289 | 5.37 | 294 | 3.64 |
– | 261 | 3.56 | 267 | 2.05 | – | – | – | – | – | – |
200 | 228 | 3.37 | 228 | 2.32 | 228 | 5.72 | 222 | 5.33 | 217 | 5.9 |
100 | – | – | – | – | – | – | – | – | – | – |
Total | 4 | 4 | 5 | 5 | 5 | |||||