Brazilian artisanal cheeses as a source of beneficial Enterococcus faecium strains: characterization of the bacteriocinogenic potential

Table 2 Primer sequences and results used in the investigation of presence of bacteriocin (enterocin A, enterocin P, enterocin B and enterocin L50B) genes in total DNA isolated from Enterococcus faecium EM485 and Enterococcus faecium EM925

Bacteriocin genes	Enterococcus faecium EM485	Enterococcus faecium EM925	Primers (5′ – 3′)	Reference
Enterocin A	+^a	+	F: GAG ATT TAT CTC CAT AAT CT	Aymerich et al. 1996
Enterocin A	+^a	+	R: GTA CCA CTC ATA GTG GAA	Aymerich et al. 1996
Enterocin P	-	-	F: ATG AGA AAA AAA TTA TTT AGT TT	Gutierrez et al. 2005
Enterocin P	-	-	R: TTA ATG TCC CAT ACC TGC CAA ACC	Gutierrez et al. 2005
Enterocin B	+	+	F: GAA AAT GAT CAC AGA ATG CCT A	Du Toit et al. 2000
Enterocin B	+	+	R: GTT GCA TTT AGA GTA TAC ATT TG	Du Toit et al. 2000
Enterocin L50B	-	-	F: ATG GGA GCA ATC GCA AAA TTA	Cintas et al. 1998
Enterocin L50B	-	-	R: TAG CCA TTT TTC AAT TTG ATC	Cintas et al. 1998

^a+ indicates positive results for genes corresponding to known bacteriocins in E. faecium EM485 and E. faecium EM925

ISSN: 1869-2044