Table 1 Sequences of primers used in quantitative real time PCR (qPCR) transcriptional analysis, with sizes of amplified fragments and annealing temperatures
Gene | Nucleotide sequence | Amplicon size (bp) | Annealing temperature (°C) |
|---|---|---|---|
agrA | 5′ CGGGTACTTGCCTGTATGAA 3′ | 149 | 58.65 |
5′ TGAATAGTTGGCGCTGTCTC 3′ | 59.03 | ||
degU | 5′ GGCGCGTATATTCATCCAC 3′ | 150 | 58.96 |
5′ TACCTCGCACTCTCTATGCG 3′ | 59.20 | ||
luxS | 5′ CATTTGATGGCAGAACTTGC 3′ | 127 | 59.28 |
5′ TGATTTCGAGTGCATCATCA 3′ | 58.73 | ||
prfA | 5′ GGAAGCTTGGCTCTATTTGC 3′ | 145 | 59.07 |
5′ ACAGCTGAGCTATGTGCGAT 3′ | 58.65 | ||
hly | 5′ AGCTCATTTCACATCGTCCA 3′ | 124 | 59.24 |
5′ TGGTAAGTTCCGGTCATCAA 3′ | 58.97 | ||
actA | 5′ AGAAATCATCCGGGAAACAG 3′ | 147 | 58.98 |
5′ CCTCTCCCGTTCAACTCTTC 3′ | 58.87 | ||
flaA | 5′ GTAAGCATCCAAGCGTCTGA 3′ | 148 | 59.03 |
5′ AAGAATCAGCATCAGCAACG 3′ | 59.03 | ||
sigB | 5′ TGGTGTCACGGAAGAAGAAG 3′ | 135 | 58.85 |
5′ TCCGTACCACCAACAACATC 3′ | 59.27 | ||
ltrC | 5′ TACGGCGTCGATGAGATACT 3′ | 144 | 58.35 |
5′ GAATGTGTGAACGGCGATAC 3′ | 59.01 | ||
sufS | 5′ GAATTTGGCGGAGAAATGAT 3′ | 137 | 58.98 |
5′ TCTGCCAAGTAATCAATCGC 3′ | 58.87 | ||
sufU | 5′ TTCAGAAATGGTGCAAGGTC 3′ | 135 | 58.70 |
5′ ATCGCTCTCTCCATTGCTTT 3′ | 59.03 |