Mapping of wine yeast and fungal diversity in the Small Carpathian wine-producing region (Slovakia): evaluation of phenotypic, genotypic and culture-independent approaches

Table 1 PCR primers used in the various culture-dependent and culture-independent approaches

Method	Designation	Sequence	Target	Reference
Real-time PCR	Hauf 2 L	CCCTTTGCCTAAGGTACG	Hanseniaspora sp.	Zott et al. 2010
	Hauf 2R	CGCTGTTCTCGCTGTGATG	Hanseniaspora sp.	Zott et al. 2010
	MPL3	CTCTCAAACCTCCGGTTTG	Metschnikowia pulcherrima	Zott et al. 2010
	MPR3	GATATGCTTAAGTTCAGCGGG	Metschnikowia pulcherrima	Zott et al. 2010
	SC1	GAAAACTCCACAGTGTGTTG	Saccharomyces sp.	Zott et al. 2010
	SC2	GCTTAAGTGCGCGGTCTTG	Saccharomyces sp.	Zott et al. 2010
	Tods L2	CAAAGTCATCCAAGCCAGC	Torulaspora delbrueckii	Zott et al. 2010
	Tods R2	TTCTCAAACAATCATGTTTGGTAG	Torulaspora delbrueckii	Zott et al. 2010
	TR03	TCTGCCCTATCAACTTTCGATGGA	Universal eukaryotic	Allmann et al. 1993
	TR04	AATTTGCGCGCCTGCTGCCTTCCTT	Universal eukaryotic	Allmann et al. 1993
Culture-independent approach and ITS sequencing	ITS1	TCCGTAGGTGAACCTGCGG	18S rRNA	White et al. 1990
	ITS3	GCATCGATGAAGAACGCAGC	5.8S rRNA	White et al. 1990
	ITS4	TCCTCCGCTTATTGATATGC	26S rRNA	White et al. 1990
	ITS4-FAM	FAM-TCCTCCGCTTATTGATATGC	26S rRNA	Brežná et al. 2010
	M13F-40	GTTTTCCCAGTCACGAC	pDrive vector	Qiagen^a
	M13R	AACAGCTATGACCATG	pDrive vector	Qiagen^a

ITS, Internal transcribed spacer
^aSequences of these primers were obtained from the Qiagen PCR cloning handbook (Qiagen, Hilden, Germany)

ISSN: 1869-2044