Table 2 Comparison of the different of approaches in terms of identification efficiency
No. of isolates analysed | Strain identity based on different methods | ||||||
|---|---|---|---|---|---|---|---|
ITS sequencinga | Real-time PCRb | Growth on lysine medium | Auxotrophy profiling | ||||
A | B | C | D | ||||
3 | DQ367881 Metschnikowia sp.: 99 % | + 1 strain of the 3 tested was positive | - | - | - | Non-Saccharomyces | Saccharomyces cerevisiae 2 Candida famata |
1 | AB469378 Saccharomycopsis vini :99 % | - | - | - | - | Non-Saccharomyces | Rhodotorula minuta |
2 | FN376418, AY027508 Pichia fermentans; FM864201 Pichia kluyveri : 99 % | - | - | - | - | Non-Saccharomyces | Candida krusei / incospicua |
6 | FJ515178, AM160628 Hanseniaspora uvarum: 100 % | - | + All 6 strains were positive | - | - | Non-Saccharomyces | Kloeckera sp. |
2 | AB469378 Torulaspora delbrueckii :99 % | - | - | - | + All 2 strains were positive | Non-Saccharomyces | Candida magnoliae |
7 | AM262829, AM262828, AM262827, AY525600 Saccharomyces cerevisiae: 100 % | + All 7 strains were positive | - | - | - | Saccharomyces | Saccharomyces cerevisiae 1 |
5 | FN393995, GQ376091, FJ793809 Saccharomyces cerevisiae, AY428861 Saccharomyces boulardii: 100 % | + All 5 strains were positive | - | - | - | Saccharomyces | Saccharomyces cerevisiae 2 |
5 | EU145764 Saccharomyces cerevisiae: 100 % | + All 5 strains were positive | - | - | - | Saccharomyces | Saccharomyces cerevisiae 2 |