Table 3 Comparison of the results obtained by ITS2 fragment sequencing and capillary electrophoresis separation of the fungal clones
Samplea | Clone name | Identity by ITS2 fragment sequencingb | Length of the amplicon by sequencing (bp) | Length of the amplicon by electrophoresis (bp) |
|---|---|---|---|---|
VG Strekov | A12 | Hanseniaspora uvarum: 100 % | 399 | 394c |
VM1 Strekov | E2 | Candida zemplinina: 100 % | 278 | 274c |
VM1 Strekov | E4 | Metschnikowia pulcherrima: 100 % | 254 | 249c |
VM1 Strekov | B13 | Aureobasidium pullulans: 100 % | 342 | 337–338c |
VM1 Strekov | B18 | Sporobolomyces marcillae: 99 % (350/355) | 394 | 390 |
VM1 Strekov | B-4 | Uncinula necator: 99 % (330/331) | 371 | 365c |
VM2 Strekov | C-6 | Saccharomyces cerevicae: 100 % | 419 | 413c |
VM2 Strekov | F1 | Candida zemplinina: 100 % | 278 | 274c |
VM2 Strekov | F4 | Candida zemplinina: 100 % | 278 | 274c |
VM2 Strekov | C8 | Sporobolomyces marcillae: 99 % (350/355) | 394 | 390 |
VG Modra | D-2 | Candida zemplinina: 100 % | 278 | 274c |
VG Modra | D-3 | Issatchenkia terricola: 100 % | 281 | 276c |
VG Modra | G29 | Candida zemplinina: 100 % | 278 | 274c |
FG Strekov | A3 | Hanseniaspora uvarum: 100 % | 399 | 393 |
FG Strekov | B2 | Metschnikowia pulcherrima: 100 % | 254 | 247 |
FG Strekov | B6 | Metschnikowia pulcherrima: 100 % | 254 | 247 |
FM2 Strekov | C1 | Saccharomyces cerevisiae: 100 % | 420 | 413 |
FM2 Strekov | C15 | Saccharomyces cerevisiae: 100 % | 420 | 413 |
FG Modra | D12 | Hanseniaspora uvarum: 99 % (357/359) | 399 | 393 |
FG Modra | D13 | Hanseniaspora uvarum: 100 % | 399 | 393 |
FG Modra | E12 | Aureobasidium pullulans: 100 % | 342 | 336 |
FM2 Modra | G3 | Torulaspora delbrueckii: 100 % | 445 | 438 |
FM2 Modra | G5 | Saccharomyces cerevisiae: 100 % | 420 | 413 |