Composition of supralittoral sediments bacterial communities in a Mediterranean island

Annals of Microbiology

Table 2 Analysis of Molecular Variance (AMOVA) of T-RFLP profiles for three localities (Lido Burrone, Praja, Faraglioni) and three environments for locality (shore-, mid-, upper-line)

Source of variation	d.f.	Sum of squares	Variance components	Percentage of variation	P-value
a) Localities
Among localities	2	25.704	0.37860	8.87	<0.2 (n.s.)
Among environments within locality	6	56.667	2.77778	65.09	<0.0001
Within locality	18	20.000	1.11111	26.04	<0.0001
Total	26	102.370	4.26749	F_ST = 0.73963
b) Environments
Among environments	2	25.926	0.37037	8.57	<0.2 (n.s.)
Among locality within environment	6	57.778	2.83951	65.71	<0.0001
Within environment	18	20.000	1.11111	25.71	<0.0001
Total	26	103.704	4.32099	F_ST = 0.71875

AMOVA was performed attributing the following two types of groupings: Computation a) is made with T-RFLP profiles grouped according to localities. Computation b) is made with T-RFLP profiles grouped according to the environment. For each grouping the percent of the total variance observed was attributed to the two hierarchical partitions. Data show the degrees of freedom (d.f.), the sum of squared deviation, the variance component estimate, the percentage of total variance contributed by each component, the F_ST statistics (Fixation index), and the probability (P) of obtaining a more extreme component estimate by chance alone estimated computing 10,000 permutations; n.s., not significant

ISSN: 1869-2044