Bacteria from spent engine-oil-contaminated soils possess dual tolerance to hydrocarbon and heavy metals, and degrade spent oil in the presence of copper, lead, zinc and combinations thereof

Table 2 Heavy metal and SEO tolerance profile of the bacterial isolates. MIC Minimal inhibitory concentration

Isolate^a	Metal MIC (μg mL^-1)^b	Time of growth in 5 % SEO (h)
Acinetobacter calcoaceticus CuD2	400	48
Achromobacter xylosoxidans CuC3	250	72
Achromobacter xylosooxidans CUE2	250	72
Acinetobacter sp. ZnE3	400	24
Acinetobacter sp. ZnB2	350	48
Arthrobacter sp. ZnC1	350	72
Acinetobacter calcoaceticus PbD1	1,100	48
Kocuria varians PbB3	1,100	72
Pseudomonas sp. PbC1	900	48
Burkholderia cepacia MD1	200	72
Kocuria varians MB2	200	48
Achromobacter xylosooxidans MC1	100	72

^aCu, Pb, Zn and M in the strain number denotes isolates showing resistance to Cu, Zn, Pb and the three metals combined
^bValues are MIC for the corresponding metals to which isolates showed resistance

ISSN: 1869-2044