Yeast species associated with spontaneous fermentation of taberna, a traditional palm wine from the southeast of Mexico

Table 2 Identification of yeast isolates by 5.8S-ITS PCR product size with three restriction endonucleases and sequencing of the D1/D2 domain of the 26S rDNA of representative strains

ITS-RFLP group	AP^a (bp)	Restriction fragment length (bp)			Sequence length^b (bp)	Species identification^c	Identity^d (%)	Accession no.
ITS-RFLP group	AP^a (bp)	HaeIII	HinfI	HhaI	Sequence length^b (bp)	Species identification^c	Identity^d (%)	Accession no.
I	850	310 + 230 + 175 + 135	360 + 360 + 130	370 + 340 + 140	559	S. cerevisiae	100	KF241567
II	470	365 + 90	255 + 215	170 + 100 + 60	563	P. kluyveri	100	KF241565
III	740	500 + 125	370 + 360	310 + 190 + 150 + 80	556	K. unispora	100	KF241561
IV	620	390 + 130 + 100	320 + 300	310 + 260	550	M. guilliermondii	100	KF241563
V	520	440 + 80	260 + 260	280 + 250	558	C. tropicalis	100	KF241559
VI	390	390	200 + 190	210 + 180	508	C.intermedia	100	KF241558
VII	750	730	340 + 220 + 190	320 + 310 + 100	550	H. guilliermondii	99	KF241560
VIII	730	475 + 240	345 + 245 + 140	360 + 280 + 75	556	K. exigua	100	KF241562
IX	535	390 + 100	225 + 160 + 150	210 + 180 + 70	598	P. kudriavzevii	98	KF241564

^aAP is the 5.8S-ITS amplified product size in base pairs (bp). ^bSequence length is the size of the D1/D2 domains of the 26S rDNA of the strains amplified with universal primers NL1 and NL4. ^cYeast species were identified by comparing the 5.8S-ITS PCR product size pattern obtained for each group with the pattern of the strains described by Esteve-Zarzoso et al. (1999), Granchi et al. (1999), Las Heras-Vazquez et al. (2003), Osorio-Cadavid et al. (2008), Stringini et al. (2008), Pham et al. (2011), and Wang and Liu (2013). ^dIdentity is the percentage of identical nucleotides in the sequence of the D1/D2 domains of 26S rDNA and the sequence with the best hit in the GenBank database. The ITS-RFLP groups are Saccharomyces cerevisiae (I), Pichia kluyveri (II), Kazachstania unispora (III), Meyerozyma guilliermondii (IV), Candida tropicalis (V), Candida intermedia (VI), Hanseniaspora guilliermondii (VII), Kazachstania exigua (VIII), and Pichia kudriavzevii (Issatchenkia orientalis) (IX).

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