Activity of acetyl-CoA carboxylase is not directly linked to accumulation of lipids when Chlorella vulgaris is co-immobilised with Azospirillum brasilense in alginate under autotrophic and heterotrophic conditions

Table 1 General analysis of data obtained from the eight experiments of immobilised Chlorella vulgaris and Azospirillum brasilense alone and combined, with and without ammonium starvation, under autotrophic and heterotrophic conditions

Lipids; per culture
Growth condition	With 90 mg NH₄ ⁺	Without NH₄ ⁺ (ammonium starvation)
Autotrophic	Comb > Chlo > Azo	Comb > Chlo > Azo
Heterotrophic	Comb > Chlo > Azo	Comb = Chlo > Azo
Best regime	Hetero > Auto	Auto > Hetero
The best treatment is co-immobilisation under autotrophic conditions and ammonium starvation
Lipids; per cell
Growth condition	With 90 mg NH₄ ⁺	Without NH₄ ⁺ (ammonium starvation)
Autotrophic	Comb > Chlo	Comb > Chlo
Heterotrophic	Comb > Chlo	Comb > Chlo
Best regime	Auto = Hetero	Auto > Hetero
The best treatment is co-immobilisation under autotrophic conditions and ammonium starvation
ACCase activity; per culture
Growth condition	With 90 mg NH₄ ⁺	Without NH₄ ⁺ (ammonium starvation)
Autotrophic	Comb > Chlo = Azo	Comb = Azo > Chlo
Heterotrophic	Comb < Chlo > Azo	Comb > Chlo > Azo
Best regime	Auto > Hetero	Auto = Hetero
The best treatment is co-immobilisation under autotrophic conditions with 90 mg NH₄ ⁺
ACCase activity; per cell
Growth condition	With 90 mg NH₄ ⁺	Without NH₄ ⁺ (ammonium starvation)
Autotrophic	Comb > Chlo	Comb > Chlo
Heterotrophic	Comb = Chlo	Comb > Chlo
Best regime	Auto > Hetero	Auto = Hetero
The best treatment is co-immobilised under autotrophic conditions with 90 mg NH₄ ⁺

Comparisons were done separately per culture or per cell for each pair of variables, using Student’s t test at p < 0.05. Azo Azospirillum brasilense, Chlo Chlorella vulgaris, Comb immobilisation of C. vulgaris with A. brasilense, Auto autotrophic growth conditions, Hetero heterotrophic growth conditions

ISSN: 1869-2044