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Table 1 Morphological characters of the bacterial isolates

From: Thermophilic bacteria that tolerate a wide temperature and pH range colonize the Soldhar (95 °C) and Ringigad (80 °C) hot springs of Uttarakhand, India

S.No.

Isolate No.

Colony and cell morphology and oxygen requirement

1

GBPI_20

Off-white, smooth, convex and round colony (dia- 0.2–0.5 mm); Gram + ve single rods; facultative anaerobic

2

GBPI_21

Off-white, irregular, convex and round colony (dia- 0.2–1.0 mm); Gram + ve diplobacilli; facultative anaerobic

3

GBPI_22

Creamish-white, smooth, convex and round colony (dia- 2.0–10 mm); Gram variable rods in long chains; facultative anaerobic

4

GBPI_23

Off-white, smooth, convex-concentric colony (dia- 5.0–10.0 mm); Gram + ve rods in short chains; aerobic

5

GBPI_24

Off-white, irregular, convex and round colony (dia-11.0–13.0 mm); Gram + ve diplobacilli; facultative anaerobic

6

GBPI_25

Creamish-white, smooth, convex and round colony (dia-1.0–2.0 mm); Gram + ve rods in long chains; facultative anaerobic

7

GBPI_26

Creamish-white, irregular, convex-raised colony (dia- 3.0–5.0 mm); Gram + ve single rods; aerobic

8

GBPI_28

Creamish-white, smooth, flat-round colony (dia- 1.5–2.0 mm); Gram variable, single-diplobacilli; aerobic

9

GBPI_30

Off-white, smooth, convex-round colony (dia- 1.0–2.0 mm); Gram + ve rods in long chains; facultative anaerobic

10

GBPI_31

Creamish-white, irregular, convex-raised colony (dia- 4.0–5.0 mm); Gram + ve rods in long chains; facultative anaerobic

11

GBPI_33

Off-white, smooth, convex-raised colony (dia- 0.2–0.5 mm); Gram + ve diplobacilli; aerobic

12

GBPI_34

Off-white, smooth, convex-raised colony (dia- 6.0–7.0 mm); Gram + ve rods in long chains with palisade arrangement; facultative anaerobic

13

GBPI_35

Creamish-white, smooth, convex-round colony (dia- 1.0–1.2 mm); Gram-variable, single rods; facultative anaerobic

14

GBPI_36

Creamish-white, smooth, convex-concentric colony (dia- 2.0–3.0 mm); Gram-variable single rods; aerobic

15

GBPI_37

Creamish-white, smooth, convex-round colony (dia- 1.0–3.0 mm); Gram-variable single rods; aerobic

16

GBPI_38

Creamish-white, smooth, convex-round colony (dia- 0.2–0.5 mm); Gram + ve single rods; facultative anaerobic

17

GBPI_39

Creamish-white, smooth, convex-round colony (dia- 0.2–1.0 mm); Gram-variable single rods; aerobic

18

GBPI_40

Brownish-white, smooth, convex- round colony (dia- 5.0–6.0 mm); Gram-variable diplobacilli; aerobic

19

GBPI_41

Off–white, smooth, convex-round colony (dia- 0.5–1.0 mm); Gram-variable diplobacilli; aerobic

20

GBPI_42

Creamish-white, smooth, convex-round colony (dia- 3.0–5.0 mm); Gram + ve single rods; aerobic

21

GBPI_43

Creamish-white, irregular, convex-round colony (dia- 0.2–3.0 mm); Gram + ve single rods; aerobic

22

GBPI_44

Off-white, smooth, convex-round colony (dia- 0.2–0.5 mm); Gram + ve diplobacilli rods; aerobic

23

GBPI_45

Brownish-white, irregular, convex-round colony (dia- 0.5–1.0 mm); Gram + ve rods in short chains; aerobic

24

GBPI_46

Creamish-white, irregular, convex-raised colony (dia- 1.0–3.0 mm); Gram-variable rods in short chains; aerobic

25

GBPI_47

Off-white, smooth, convex-round colony (dia- 0.5–1.0); Gram-variable rods in short chains; aerobic

26

GBPI_48

Off-white, smooth, convex-round colony (dia-1.3–2.5 mm); Gram-variable diplobacilli rods; aerobic

27

GBPI_50

Off-white, smooth, convex-round colony (dia- 0.5–1.0 mm); Gram-variable rods in long chains; aerobic

28

GBPI_52

Brownish-white, irregular, convex-raised colony (2.0 mm); Gram-variable rods in shorts chain; aerobic