Thermophilic bacteria that tolerate a wide temperature and pH range colonize the Soldhar (95 °C) and Ringigad (80 °C) hot springs of Uttarakhand, India

Table 2 Temperature and pH range and enzymatic activities of the bacterial isolates

S.No.	Isolate No.	Temperature (°C)	pH	Enzymatic activities
S.No.	Isolate No.	Temperature (°C)	pH	Amylase	Protease	Lipase	Cellulase	Xylanase
1	GBPI_20	20 to 80	5–14	+	+	+	–	+
2	GBPI_21	20 to 80	5–14	+	+	+	+++	–
3	GBPI_22	20 to 70	4–14	+++	+	+	+++	–
4	GBPI_23	20 to 65	5–14	+	+	+	+	–
5	GBPI_24	20 to 70	4–14	+	+	+	+	–
6	GBPI_25	20 to 70	5–14	+	+	+	+++	+++
7	GBPI_26	20 to 70	5–14	+	+	+	+	+
8	GBPI_28	20 to 65	5–14	–	–	–	+	–
9	GBPI_30	20 to 70	5–14	+	–	+	+	–
10	GBPI_31	20 to 80	5–14	+	+	+	+	–
11	GBPI_33	20 to 65	5–14	+	–	+	+	–
12	GBPI_34	20 to 70	5–14	+	+	+	++	–
13	GBPI_35	20 to 80	5–14	+	+	+	+	–
14	GBPI_36	20 to 70	5–14	+	+	+	+	+
15	GBPI_37	20 to 70	5–14	+	+	+	+	–
16	GBPI_38	20 to 70	5–14	+	+	+	++	+
17	GBPI_39	20 to 70	5–14	+	+	+	+	–
18	GBPI_40	20 to 70	4–14	+	–	+	+	+
19	GBPI_41	20 to 80	4–14	+	+	+	+	–
20	GBPI_42	20 to 80	5–14	+	+	+	+	+
21	GBPI_43	20 to 80	5–14	+	+	+	+	+
22	GBPI_44	20 to 65	5–14	+	+	+	–	–
23	GBPI_45	20 to 80	5–14	+	+	+	–	+
24	GBPI_46	20 to 80	5–14	+	+	+	+	+
25	GBPI_47	20 to 65	5–14	+	–	+	+	+
26	GBPI_48	20 to 70	5–14	+	+	+	+	+
27	GBPI_50	20 to 80	5–14	–	–	–	–	–
28	GBPI_52	20 to 80	4–14	–	+++	–	+	+

Optimum temperature and pH for all the bacterial isolates: 55–65 °C and 7, respectively; +++ = strong (>5.0 mm); ++ = moderate (2.0–5.0 mm); + weak (<2.0 mm)

ISSN: 1869-2044