Fig. 3
From: Pterostilbene production by microorganisms expressing resveratrol O-methyltransferase
a–c HPLC analysis of resveratrol, pinostilbene and pterostilbene produced by heterologous recombinant E. coli and Saccharomyces cerevisiae. a HPLC profiles of four authentic standards of p-coumaric acid, resveratrol, pinostilbene and pterostilbene with retention times of 9.162, 11.863, 15.163 and 18.757 min, respectively. b HPLC profiles of extracts from E. coli and S. cerevisiae cells expressing the ROMT gene. E. coli and S. cerevisiae cells containing pTrc-ROMT or pAG305GPD-ROMT were cultured as described in “Materials and methods”. c HPLC profiles of extracts from E. coli and S. cerevisiae cells co-expressing the 4CL::STS and ROMT genes. HPLC chromatograms showed the production of resveratrol and pterostilbene from p-coumaric acid in both microorganisms harboring either pAG304GPD-4CLSTS and pAG305GPD-ROMT or both plasmids pAC-4CLSTS and pTrc-ROMT