Table 1 Primer pairs used in this study. F Forward, R reverse
From: Pterostilbene production by microorganisms expressing resveratrol O-methyltransferase
Primer | Sequence (5′-3′) |
|---|---|
RMOT-F | ATG GAT TTG GCA AAC GGT GTG ATA TC |
RMOT-R | TCA AGG ATA AAC CTC AAT GAG GGA CC |
SumoROMTF | CGC GAA CAG ATT GGA GGT GAT TTG GCA AAC GGT GTG ATA TCA GC |
SumoROMTR | GTG GCG GCC GCT CTA TTA TCA AGG ATA AAC CTC AAT GAG GGA CC |
4CL-F | ATG GCG CCA CAA GAA CAA GCA GTT TC |
STS-R | TTA ATT TGT AAC CAT AGG AAT GCT ATG |
4CL-XbaIF | GCT CTA GAA GGA GGA TTA CAA AAT GGC GCC ACA AGA ACA AGC AGT TTC TC |
STS-NotIR | TTG CGG CCG CTT AAT TTG TAA CCA TAG GAA TGC TAT G |
ROMT-BamHIF | CGG GAT CCA TGG ATT TGG CAA ACG GTG TGA TAT C |
ROMT-NotIR | TTG CGG CCG CTC AAG GAT AAA CCT CAA TGA GGG ACC |
Oligo dT (22) | TTT TTT TTT TTT TTT TTT TTV N |
167F | GCT CAA CAA TTC CGC AAA TGA AGC CAT GGC |
167R | GCC ATG GCT TCA TTT GCG GAA TTG TTG AGC |
174F | GCC ATG GCT AGC GCA GCT CGC TTA CTC AC |
174R | GTG AGT AAG CGA GCT GCG CTA GCC ATG GC |
258F | CAA TTT TAC TCA AGG CAA TAC TGC ACG AC |
258R | GTC GTG CAG TAT TGC CTT GAG TAA AAT TG |
261F | CAA GTG GAT ACT GGC AGA CTG GAG CGA TG |
261R | CAT CGC TCC AGT CTG CCA GTA TCC ACT TG |