Table 1 Effects of different culture conditions on dry cell weight, NADH oxidase activity and hydrogen peroxide accumulation in BHI medium at 25 °C after a 24-h incubation with Enterococcus italicus strain TP1.5T and Lactococcus garvieae strains TB25 and LG9
Bacterial strain | Growth conditions | DCW ± SD (mg l−1) | NADH oxidase activity (U mg−1) | Hydrogen peroxide (mmol l−1) | |||
|---|---|---|---|---|---|---|---|
2 h | 4 h | 6 h | 8 h | ||||
Enterococus italicus strain TP1.5T | Anaerobic | 131 ± 7 | 0 | 0 | 0 | 0 | 0 |
Aerobic | 135 ± 6.8 | 10.3 ± 0.5 | 1.2 ± 0.1 | 2.1 ± 0.2 | 3.5 ± 0.3 | 4.9 ± 0.4 | |
Aerobic + FADa | 129 ± 6.5 | 62.1 ± 3.1 | np | np | np | np | |
Lactococcus garvieae strain TB25 | Anaerobic | 142 ± 7.1 | 0 | 0 | 0 | 0 | 0 |
Aerobic | 148 ± 7.4 | 7.1 ± 0.4 | 0.3 ± 0.0 | 0.5 ± 0.0 | 0.7 ± 0.0 | 0.9 ± 0.0 | |
Aerobic + FADa | 139 ± 7 | 7.3 ± 0.4 | 0.3 ± 0.0 | 0.4 ± 0.0 | 0.6 ± 0.0 | 0.9 ± 0.0 | |
Lactococcus garvieae strain LG9 | Anaerobic | 167 ± 8.4 | 0 | 0 | 0 | 0 | 0 |
Aerobic | 171 ± 8.5 | 6.1 ± 0.3 | 0.3 ± 0.0 | 0.6 ± 0.0 | 0.9 ± 0.0 | 1.1 ± 0.1 | |
Aerobic + FADa | 163 ± 8.2 | 6.4 ± 0.3 | 0.3 ± 0.0 | 0.4 ± 0.0 | 0.6 ± 0.0 | 0.9 ± 0.1 | |